Fig. 4

Phenotypic and functional analysis of ASC-educated M2 Mphs. Flow cytometry analysis of a phagocytosis levels of Zymosan A, E. coli, or S. aureus particles labeled with pHrodo™. Average ± SEM of positive percentages and Geo Mean statistics are shown below; b surface expression of several phagocytic receptors; c surface expression of co-stimulatory molecules; d surface chemokine receptors, in M2 Mphs, in the presence or absence of ASCs; and e Average ± SEM of positive percentages and Geo Mean of the different surface markers. f OLINK analysis of the secretome of M2 Mphs alone and ASC-M2 Mphs co-cultures. Data are representative of at least four independent experiments. The table shows the fold change of NPX between ASC-M2 Mphs and M1 alone, together with the p value for this calculation; red indicates the downregulation of targets; only statistically significant changes are shown (n = 4). *p < 0.05. ASC, adipose-derived mesenchymal stem cell; CCR, C-C motif chemokine receptor; CD, cluster of differentiation; CX3CR, CX3C chemokine receptor; CXCR, C-X-C chemokine receptor; E. coli, Escherichia coli; HLA, human leukocyte antigen; IL, interleukin; Mph, macrophage; NPX, normalized protein expression; OPG, osteoprotegerin; S. aureus, Staphylococcus aureus