Fig. 5

Phenotypic and functional analysis of ASC-educated mDCs. Flow cytometry analysis of a surface expression of CD14/CD1a; b phagocytosis levels of Zymosan A, E. coli, or S. aureus particles labeled with pHrodo™. Average ± SEM of positive percentages and Geo Mean statistics are shown below; c surface expression of several phagocytic receptors; d surface expression of co-stimulatory molecules; and e surface chemokine receptors in mDCs in the presence or absence of ASCs. f Average ± SEM of positive percentages and Geo Mean of the different surface markers. Data are representative of at least four independent experiments; g OLINK analysis of the secretome of mDCs alone and ASC–mDC co-cultures. The table shows fold change of NPX between ASC–mDC and mDCs alone, together with the p value for this calculation; green indicates the upregulation of targets, and red indicates the downregulation of targets; only statistically significant changes are shown (n = 4). *p < 0.05, **p < 0.01. ASC, adipose-derived mesenchymal stem cell; CCL, C-C motif chemokine; CCR, C-C motif chemokine receptor; CD, cluster of differentiation; CX3CR, CX3C chemokine receptor; CXCL, C-C-C motif chemokine; CXCR, C-X-C chemokine receptor; E. coli, Escherichia coli; EN.RAGE, protein S100-A12; HLA, human leukocyte antigen; IL, interleukin; mDC, mature dendritic cell; NPX, normalized protein expression; NT-3, neurotrophin-3; OSM, oncostatin-M; S. aureus, Staphylococcus aureus; TNF, tumor necrosis factor; TNFSF, tumor necrosis factor ligand superfamily member