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Fig. 8 | Stem Cell Research & Therapy

Fig. 8

From: Human adipose mesenchymal stem cells modulate myeloid cells toward an anti-inflammatory and reparative phenotype: role of IL-6 and PGE2

Fig. 8

CD14/CD1a surface expression, phagocytic capacity, and CD163 expression of mDCs differentiated with ASC-conditioned supernatant. a PGE2 ELISA of basal or IL-1β-stimulated ASC-conditioned media. b Average positive percentage ± SEM of CD14+/CD1a mDC differentiated with basal or IL-1β-conditioned media (n = 3). c Average positive percentage increase of S. aureus phagocytosis versus mDC alone ± SEM of mDC differentiated with basal or IL-1β-conditioned media (n = 3). d Dot plots showing the surface expression of CD14/CD1a, in monocyte-derived mDCs in the presence of basal or IL-1β-conditioned media, measured by flow cytometry (data are representative of 3 independent experiments). e Histograms showing the phagocytosis levels of S. aureus by monocyte-derived mDCs, in the presence of basal or IL-1β-conditioned media, measured by flow cytometry (data are representative of 3 independent experiments). f Surface expression levels of CD163 on mDC differentiated in the presence of basal or IL-1β-conditioned media, measured by flow cytometry (data are representative of 3 independent experiments). ***p < 0.001. ASC, adipose-derived mesenchymal stem cell; CD, cluster of differentiation; CM, conditioned media; IL, interleukin; mDC, mature dendritic cell; PGE2, prostaglandin E2; S. aureus, Staphylococcus aureus

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