Table 1 qPCR primer sequences
Gene | Forward primer | Reverse primer |
|---|---|---|
TJP1 | CAACATACAGTGACGCTTCACA | CACTATTGACGTTTCCCCACTC |
TJP2 | TTTCCCGACACCCCAAAGTAG | CACCCTGCTTCCTTGAGACC |
TJP3 | AGCTACAAGCCTCGGGTTCC | GCTGTTCCCAGATGGTCAGC |
JAM1 | ATGGGGACAAAGGCGCAAG | CAATGCCAGGGAGCACAACA |
JAM3 | TCCAGCAATCGAACCCCAG | CTTGTCTGCGAATCCGTAATGAT |
CLDN5 | CTCTGCTGGTTCGCCAACAT | CAGCTCGTACTTCTGCGACA |
CLDN6 | TGTTCGGCTTGCTGGTCTAC | CGGGGATTAGCGTCAGGAC |
CLDN7 | AGTTAGGAGCCTTGATGCCG | GCACAGGGAGTAGGATACGC |
CLDN11 | CATTGCCAGTTGACTGCCTG | ATCCGGATGCAGGGAAGAAC |
ACTN2 | GACATCGTGAACACCCCTAAAC | CCGCAAAAGCGTGGTAGAA |
E-cadherin | ACCACGGGCTTGGATTTTGA | GGAGGTGGTGAGAGAGACCT |
ROCK1 | AAGTGAGGTTAGGGCGAAATG | AAGGTAGTTGATTGCCAACGAA |
RUNX1 | GTGGGTACGAAGGAAATGACTCAAA | GCAGCGTGGTAAAAGAAATCATTGAG |
GAPDH | CCATGTTCGTCATGGGTGTGA | CATGGACTGTGGTCATGAGT |