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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Congenital muscular dystrophy-associated inflammatory chemokines provide axes for effective recruitment of therapeutic adult stem cell into muscles

Fig. 5

Systemic transplantation of selected ADSC into Col6a1−/− mice. a Representative in vivo images showing the hindlimbs of mice receiving Ccc2-positive ADSC and Cxcr2-positive ADSC transplants under physiological (−CTX) and pro-inflammatory (+CTX) conditions, respectively. IVIS live imaging was performed at 4, 14, and 21 days post-transplant. b Quantitative analysis of fluorescence detected by live imaging from differently treated cohorts of mice (n = 3 per time point) at 3 time points (as indicated below the columns). Data are presented as radiant efficacy ± SD and as fluorescent area ± SD, respectively. Color-coding for Ccr2-positive ADSC and Cxcr2-positive ADSC is shown in the key. Dotted trend lines illustrate time-dependent changes in differently treated mice, as indicated in the key. Statistically significant differences in Ccr2- and Cxcr2-positive ADSC-treated mice (p < 0.05) are indicated with asterisks (***). c Immunofluorescent analysis of GCM tissue from untreated and CTX-treated muscle biopsies was performed 21 days after transplantation. Co-localization of the ADSC-donated COL6 and basement membrane-associated type IV collagen was detected with α1(VI) collagen (AlexaFluor488, green) and LAMA2 (AlexaFluor594, red) antibodies. Images were taken from representative sections at low and high (20x) magnification, respectively. Nuclei were stained with DAPI (blue). Scale bar, 100 μm (low magnification) and 25 μm (high magnification), respectively. d Quantitative assessment of COL6-positive myofibers on sections of muscle tissue from mice treated with unselected ADSC, Ccr2-positive ADSC, and Cxcr2-positive ADSC, respectively. Data are presented as the percentage of COL6-positive myofibers per microscopic field ± SD. Time points of tissue collection and treatments are indicted below the columns. Statistical significance (p < 0.05) is indicated with asterisks (***)

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