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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Investigation of de novo mutations in a schizophrenia case-parent trio by induced pluripotent stem cell-based in vitro disease modeling: convergence of schizophrenia- and autism-related cellular phenotypes

Fig. 1

Establishment and molecular characterization of NPC lines and mature neuronal cultures. Investigation of target genes KHSRP and LRRC7. a Changes in gene expression patterns in NPCs and neurons derived from the case-parent trio. b, c NPCs and neurons derived from hiPSCs by the hippocampal neuronal differentiation protocol were investigated by immunofluorescence staining and visualized by confocal fluorescent microscopy. Immunocytochemical staining shows Nestin/Sox2 (b) and Map 2/Prox1 (c) positivity in these established neural cell types. Scale bars = 100 μm. d, e Immunofluorescence staining for KHSRP and LRRC7 in neurons. KHSRP (d) shows nuclear and cytoplasmic localization, while LRRC7 (e) localized postsynaptically in neurons. Scale bars = 50 μm

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