Fig. 3

Investigation of calcium-activity in NPCs. a Normalized Ca²⁺ activity (F/F_baseGlu) for one representative measurement of NPCs representing each subject. On the upper part of the subplot the Y-axis represents the mean activity (+/− standard deviation, shattered blue) and X-axis represents time. On the lower part, Y-axis represents the activity of all cells and X-axis represents time. Color indicates Ca²⁺ activity. b Each circle represents the normalized Ca²⁺ activity of one cell for each subject in each clone (N = 3 independent experiments, n = 200–250 cells/experiments). Black diamonds indicate the mean activity of a subject for both clones, while the error bars indicate one standard deviation. Red circles indicate NPCs derived from iPSC clone 1, blue circles NPCs derived from iPSC clone 2. For time series and heatmap images of clones 2, see Supplementary Fig. 3