Table 1 Primer sequences using reverse transcription polymerase chain reaction
Genes | Primer sequences | Tm | |
|---|---|---|---|
Oct4 | Forward | 5′-AGTGAGAGGCAACCTGGAGA-3’ | 52 |
Reverse | 5′-GTGAAGTGAGGGCTCCCATA-3’ | ||
Nanog | Forward | 5′-TTCTTGACTGGGACCTTGTC-3’ | 52 |
Reverse | 5′-GCTTGCCTTGCTTTGAAGCA-3’ | ||
Sox2 | Forward | 5′-GGGCAGCGTGTACTTATCCT-3’ | 52 |
Reverse | 5′-AGAACCCCAAGATGCACAAC-3’ | ||
HLA-G | Forward | 5′-GCGGCTACTACAACCAGAGC-3’ | 58 |
Reverse | 5′-GCACATGGCACGTGTATCTC-3’ | ||
TERT | Forward | 5′-GAGCTGACGTGGAAGATGAG-3’ | 55 |
Reverse | 5′-CTTCAAGTGCTGTCTGATTCCAATG-3’ | ||
AFP | Forward | 5′-ATGCTGCAAACTGACCACGC-3’ | 55 |
Reverse | 5′-GCTTCGCTTTGCCAATGCTT-3’ | ||
Albumin | Forward | 5′-TGAGTTTGCAGAAGTTTCCA-3’ | 60 |
Reverse | 5′-CCTTTGCCTCAGCATAGTTT-3’ | ||
β-actin | Forward | 5′-TCCTTCTGCATCCTGTCAGCA-3’ | 58 |
Reverse | 5′-CAGGAGATGGCCACTGCCGCA-3’ |