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Table 2 Primer sequences using quantitative real time polymerase chain reaction

From: Functionally enhanced placenta-derived mesenchymal stem cells inhibit adipogenesis in orbital fibroblasts with Graves’ ophthalmopathy

Genes   Primer sequences Tm
OC Forward 5′-AGTGAGAGGCAACCTGGAGA-3’ 52
Reverse 5′-GTGAAGTGAGGGCTCCCATA-3’
COL1A1 Forward 5′-TTCTTGACTGGGACCTTGTC-3’ 52
Reverse 5′-GCTTGCCTTGCTTTGAAGCA-3’
Adipsin Forward 5′-GGGCAGCGTGTACTTATCCT-3’ 52
Reverse 5′-AGAACCCCAAGATGCACAAC-3’
PPAR-γ Forward 5′-GCGGCTACTACAACCAGAGC-3’ 58
Reverse 5′-GCACATGGCACGTGTATCTC-3’
Adiponectin Forward 5′-GAGCTGACGTGGAAGATGAG-3’ 55
Reverse 5′-CTTCAAGTGCTGTCTGATTCCAATG-3’
Leptin Forward 5′-ATGCTGCAAACTGACCACGC-3’ 55
Reverse 5′-GCTTCGCTTTGCCAATGCTT-3’
LPL Forward 5′-TGAGTTTGCAGAAGTTTCCA-3’ 60
Reverse 5′-CCTTTGCCTCAGCATAGTTT-3’
FABP4 Forward 5′-GCATGGCCAAACCTAACATGA-3’ 55
Reverse 5′-CCTGGCCCAGTATGAAGGAAA-3’
IGFBP1 Forward 5′-GAGCCCTGCCGAATAGAAC-3’ 60
Reverse 5′-GGATCCTCTTCCCATTCCAAG-3’
IGFBP2 Forward 5′-ACATCCCCAACTGTGACAAG-3’ 60
Reverse 5′-ATCAGCTTCCCGGTGTTG-3’
IGFBP3 Forward 5′-CAGAGCACAGATACCCAGAAC-3’ 60
Reverse 5′-AGCACATTGAGGAACTTCAGG-3’
IGFBP4 Forward 5′-CTGACAGCTTTCGAGAGTGAG-3’ 60
Reverse 5′-GCGCATTTGAGGGAAACTTC-3’
IGFBP5 Forward 5′-ACCCAGTCCAAGTTTGTCG-3’ 60
Reverse 5′-TGTAGAATCCTTTGCGGTCAC-3’
IGFBP6 Forward 5′-GTCTACACCCCTAACTGCG-3’ 60
Reverse 5′-CTCTGTTGGTCTCTGCGG-3’
IGFBP7 Forward 5′-GCCCAGAAAAGCATGAAGTAAC-3’ 60
Reverse 5′-TTTATAGCTCGGCACCTTCAC-3’
ITGA4 Forward 5′-AGAGAGACAATCAGTGGTTGG-3’ 55
Reverse 5′-TCAGTTCTGTTCGTAAATCAGG-3’
ITGB7 Forward 5′-AGCAGCAACAACTCAACTGG-3’ 55
Reverse 5′-TTACAGACCCACCCTTCCTCT-3’
FAK Forward 5′-GAAGCATTGGGTCGGGAACTA-3’ 55
Reverse 5′-CTCAATGCAGTTTGGAGGTGC-3’
GAPDH Forward 5′-TCCTTCTGCATCCTGTCAGCA-3’ 58
Reverse 5′-CAGGAGATGGCCACTGCCGCA-3’