Table 2 Primer sequences using quantitative real time polymerase chain reaction
Genes | Primer sequences | Tm | |
|---|---|---|---|
OC | Forward | 5′-AGTGAGAGGCAACCTGGAGA-3’ | 52 |
Reverse | 5′-GTGAAGTGAGGGCTCCCATA-3’ | ||
COL1A1 | Forward | 5′-TTCTTGACTGGGACCTTGTC-3’ | 52 |
Reverse | 5′-GCTTGCCTTGCTTTGAAGCA-3’ | ||
Adipsin | Forward | 5′-GGGCAGCGTGTACTTATCCT-3’ | 52 |
Reverse | 5′-AGAACCCCAAGATGCACAAC-3’ | ||
PPAR-γ | Forward | 5′-GCGGCTACTACAACCAGAGC-3’ | 58 |
Reverse | 5′-GCACATGGCACGTGTATCTC-3’ | ||
Adiponectin | Forward | 5′-GAGCTGACGTGGAAGATGAG-3’ | 55 |
Reverse | 5′-CTTCAAGTGCTGTCTGATTCCAATG-3’ | ||
Leptin | Forward | 5′-ATGCTGCAAACTGACCACGC-3’ | 55 |
Reverse | 5′-GCTTCGCTTTGCCAATGCTT-3’ | ||
LPL | Forward | 5′-TGAGTTTGCAGAAGTTTCCA-3’ | 60 |
Reverse | 5′-CCTTTGCCTCAGCATAGTTT-3’ | ||
FABP4 | Forward | 5′-GCATGGCCAAACCTAACATGA-3’ | 55 |
Reverse | 5′-CCTGGCCCAGTATGAAGGAAA-3’ | ||
IGFBP1 | Forward | 5′-GAGCCCTGCCGAATAGAAC-3’ | 60 |
Reverse | 5′-GGATCCTCTTCCCATTCCAAG-3’ | ||
IGFBP2 | Forward | 5′-ACATCCCCAACTGTGACAAG-3’ | 60 |
Reverse | 5′-ATCAGCTTCCCGGTGTTG-3’ | ||
IGFBP3 | Forward | 5′-CAGAGCACAGATACCCAGAAC-3’ | 60 |
Reverse | 5′-AGCACATTGAGGAACTTCAGG-3’ | ||
IGFBP4 | Forward | 5′-CTGACAGCTTTCGAGAGTGAG-3’ | 60 |
Reverse | 5′-GCGCATTTGAGGGAAACTTC-3’ | ||
IGFBP5 | Forward | 5′-ACCCAGTCCAAGTTTGTCG-3’ | 60 |
Reverse | 5′-TGTAGAATCCTTTGCGGTCAC-3’ | ||
IGFBP6 | Forward | 5′-GTCTACACCCCTAACTGCG-3’ | 60 |
Reverse | 5′-CTCTGTTGGTCTCTGCGG-3’ | ||
IGFBP7 | Forward | 5′-GCCCAGAAAAGCATGAAGTAAC-3’ | 60 |
Reverse | 5′-TTTATAGCTCGGCACCTTCAC-3’ | ||
ITGA4 | Forward | 5′-AGAGAGACAATCAGTGGTTGG-3’ | 55 |
Reverse | 5′-TCAGTTCTGTTCGTAAATCAGG-3’ | ||
ITGB7 | Forward | 5′-AGCAGCAACAACTCAACTGG-3’ | 55 |
Reverse | 5′-TTACAGACCCACCCTTCCTCT-3’ | ||
FAK | Forward | 5′-GAAGCATTGGGTCGGGAACTA-3’ | 55 |
Reverse | 5′-CTCAATGCAGTTTGGAGGTGC-3’ | ||
GAPDH | Forward | 5′-TCCTTCTGCATCCTGTCAGCA-3’ | 58 |
Reverse | 5′-CAGGAGATGGCCACTGCCGCA-3’ |