Fig. 2

DOX-hLIF-2i piPSCs were characteristically pluripotent. a Schematic illustration of DOX-hLIF-2i piPSCs generation and cell morphologies from P0 to P21. b AP staining of DOX-hLIF-2i piPSCs. Scale bar, 200 μm. c qRT-PCR analysis of pluripotency-associated gene expression. 1# and 2# represent two lines of DOX-hLIF-2i piPSCs. FRT-9 represents naive-like piPSCs derived by Zhang et al. [15]. Comparisons of multiple groups were analyzed using one-way ANOVA with Tukey’s multiple comparison test. Groups with different letters indicate significant differences (p < 0.05). d Immunofluorescence assay of OCT4, SOX2, and NANOG. Scale bar, 20 μm. e Immunofluorescence assay of 3-germ-layer cells in EBs. Scale bar, 100 μm. f Karyotype analysis of DOX-hLIF-2i piPSCs. g qRT-PCR analysis of IRF-1 in DOX-hLIF-2i piPSCs and PEFs