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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: IRF-1 expressed in the inner cell mass of the porcine early blastocyst enhances the pluripotency of induced pluripotent stem cells

Fig. 4

High-level expression of IRF-1was associated with higher expression of naïve pluripotency-associated genes in DOX-hLIF-2i piPSCs. a detection of IRF-1-GFP in DOX-hLIF-2i piPSCs. Scale bars from left to right, 200 μm, 20 μm. b FACS analysis of GFP-positive cells in DOX-hLIF-2i piPSCs. c qRT-PCR analysis of genes in the GFP-positive and -negative cells. **p < 0.01; ***p < 0.001. d AP staining of GFP-positive and -negative cells. GFP- represents GFP negative cells and GFP+ represents GFP positive cells

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