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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: IRF-1 expressed in the inner cell mass of the porcine early blastocyst enhances the pluripotency of induced pluripotent stem cells

Fig. 5

ChIP-Seq analysis for genes targeted by IRF-1. a Immunofluorescence co-localization of IRF-1 with FLAG antibody. IRF-1 was overexpressed fusion with 3× Flag. 3× Flag was alone expressed as a control. Scale bar, 20 μm. b The profiles of peak signals of IRF-1-Flag. Signals are shown for 10 kb up- and downstream of the TSS. c KEGG pathways enriched from IRF-1 targeted sites. d Peaks related to IL7, STAT3. Flag denotes signals detected with the anti-flag antibody in IRF-1-Flag overexpressing cells. e qRT-PCR analysis of genes in the GFP-positive and -negative cells. *p < 0.05; **p < 0.01; ***p < 0.001. f qRT-PCR analysis of genes in the IRF-1 overexpressing cells and control cells. *p < 0.05; **p < 0.01; ***p < 0.001. f The expression of genes in the ICM and TE. **p < 0.01. The rpkm was analyzed from transcriptome data sequenced by Liu group. h qRT-PCR of genes in IRF-1-overexpressing cells treated with Stattic. NC represents control piPSCs. OE represents IRF-1-overexpressing cells. OE+Stattic represents IRF-1-overexpressing cells treated with stattic for 3 days. Comparisons of multiple groups were analyzed using one-way ANOVA with Tukey’s multiple comparison test. Groups with different letters indicate significant differences (p < 0.05)

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