Fig. 6

IRF-1 is a positive regulator for reprogramming. a–c The impact of IRF-1 knockdown on reprogramming. d–f Impact of IRF-1 overexpression during reprogramming. g–j The detection of pluripotency in PMX-POKSMI piPSCs. a, d qRT-PCR analysis of IRF-1 expression at day 5 of reprogramming. b, e Number of AP+ colonies at day 20 of reprogramming. The numbers are presented as mean values ± SD from 3 independent experiments. c, f AP+ colonies of pre-piPSCs at day 20 of reprogramming. Scale bar, 5 mm. g Morphologies of OKSMI piPSCs from P0 to P3. Scale bar, 200 μm. h AP staining of OKSMI piPSCs. Scale bar, 200 μm. i qRT-PCR analysis of pluripotency-associated genes of OKSMI piPSCs. 4# and 10# represent different lines of OKSMI piPSCs. The control represents OKSM piPSCs. Comparisons of multiple groups were analyzed using one-way analysis ANOVA with Tukey’s multiple comparison test. Groups with different letters indicate significant differences (p < 0.05). j Immunofluorescence assay of OCT4, SOX2, NANOG. Scale bar, 20 μm