Fig. 3
Melphalan treatment of hiPSC-CMs alters the expression of proteins identified by proteomic analysis. Proteomic analysis of hiPSC-CMs treated with 0 and 20 μM melphalan for 3 days (n = 3). a Volcano plot illustrating proteins with statistically significant differences in their abundance between control and melphalan-treated hiPSC-CMs. The log2(fold change) was plotted on the x-axis and the −log10(P value) on the y-axis (P value < 0.05 and fold change > 1.5). b Venn diagram showing the numbers of differentially expressed proteins identified by proteomics (purple circle) and genes identified by RNA-Seq (green circle). The red line divides areas into upregulated part and downregulated part. c Bar charts showing up- and downregulated proteins clustered by GO enrichment analysis. Length of bar indicates −log10(P value), and value of n denotes the count of involved proteins in each term. Control, no melphalan; Mel, 20 μM melphalan