Fig. 6

NAC ameliorates the alteration of cardiomyocyte beating indexes caused by melphalan. Analysis of hiPSC-CM contractility upon melphalan treatment with or without NAC supplementation for 3 days. a Representative traces showing the beating velocity recording of hiPSC-CMs. Blue dots denote contraction, and red triangles denote relaxation. b Quantification of maximum contraction, maximum relaxation, and beating interval changes. Relative values were calculated by dividing by the average beating velocity of no melphalan treatment groups, respectively (sample sizes were the same as c). c Stacked bar charts showing the percentage of wells of cells with regular (blue) or irregular (red) contractility pattern. Sample sizes (n) were given at the top of each bar. Comparisons were done between the groups indicated via one-way ANOVA test and two-tailed Student’s t test for b, or two-sided chi-square test for c. *P value < 0.05; **P value < 0.01; ***P value < 0.001; ****P value < 0.0001