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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Melphalan induces cardiotoxicity through oxidative stress in cardiomyocytes derived from human induced pluripotent stem cells

Fig. 7

NAC attenuates melphalan-induced alteration of hiPSC-CM transcriptome profiles characterized by RNA-Seq analysis. RNA-Seq analysis of hiPSC-CMs upon 0 and 20 μM melphalan treatment with or without NAC supplementation for 3 days (n = 3). a Volcano plots presenting the DEGs when comparing any two groups. The up- or downregulated genes were identified based on padj < 0.01 and fold change > 2. b Bar charts showing top 20 downregulated GO terms in melphalan-treated hiPSC-CMs compared with control group, and the enrichment results of these GO terms in Mel+NAC-treated hiPSC-CMs compared with melphalan group. Length of bar indicates −log10(padj), and the value of n denotes the count of involved genes in each term. c Chord diagrams showing the DEGs of interested KEGG clusters in melphalan-treated hiPSC-CMs compared with control group, and the relative expression of these genes in Mel+NAC-treated hiPSC-CMs compared with melphalan group. In each chord diagram, KEGG pathways were presented on the right, and genes contributing to these enrichments were drawn on the left. Blue and red colors of displayed squares on the left indicate the levels of gene expression according to log2(fold change). The dark orange dashed lines were the boundary between up- and downregulated genes. d Heatmap showing the DEGs involved in GO terms of oxidative stress and cardiac muscle contraction in melphalan- or Mel+NAC-treated hiPSC-CMs compared with control group. Blue and red colors of displayed rectangles indicate the levels of gene expression according to log2(fold change). padj, adjusted P value; Control, no melphalan; Mel, 20 μM melphalan; Mel+NAC, 20 μM melphalan with 1 mM NAC

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