Fig. 3

Gal-9-ERC promoted Tregs while inhibiting B cell activation in vitro. On account of the above co-culture experiments, total cell mRNA was extracted and used for RT-PCR analysis. IFN-γ (a), IL-17 (b), and EBI3 (IL-35) (c) mRNA relative expression differences were measured among different groups respectively (n = 6). d Representative dot plots of Treg (CD4⁺CD25⁺Foxp3⁺) cells, and the stimulant for Tregs was IL-2 + anti-CD3/CD28 Abs. Gating strategy was shown above, and all the following analysis were gated on CD4⁺ population. e Representative dot plots of activated B (CD19⁺CD86⁺) cells and stimulant for B cells is LPS. f, g Percentage of Treg (CD4⁺CD25⁺Foxp3⁺) and B (CD19⁺CD86⁺) cells (n = 6). h, i Furthermore, to evaluate antibody-producing plasma B cell levels, IgM (h) and IgG (i) in the culture supernatant were also collected and detected by ELISA (n = 6). Differences among groups were assessed by using one-way analysis of variance (ANOVA). *p < 0.05, **p < 0.01. Abbreviations: ERC, endometrial regenerative cell; Gal-9-ERC, Galectin-9 high-expression ERC; EBI3, Epstein-Barr Virus Induced 3; LPS, lipopolysaccharides