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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Improving cell survival and engraftment in vivo via layer-by-layer nanocoating of hESC-derived RPE cells

Fig. 3

Morphological and functional assessment of untreated RPE and LbL-RPE cells in vitro. a Differences in F-actin (green) morphology in cultures of untreated RPE and LbL-RPE cells stained with phalloidin indicate there were no differences between untreated and treated cells on days 6, 9, and 30 (cell nuclei—blue Hoechst stain). Scale bar 20 μm. b Immunostaining for PEDF (green) in untreated RPE and LbL-RPE cells was used as a marker to demonstrate continued presence of PEDF on days 6, 9, and 30 (cell nuclei—blue Hoechst stain). Scale bar 20 μm. c PEDF release curves for untreated RPE and LbL-RPE cells. Cells were cultured at pH 6.5–7.45 in 12-well plates. PEDF concentration in the medium was tested with an ELISA kit. Data represent the mean ± SEM of three independent experiments (4 to 30 days in culture; p > 0.05). d Measurement of ability to phagocytose photoreceptor outer segments (POS) by RPE and LbL-RPE cells. RPE and LbL-RPE cells were cultured with FITC-POS at 37 °C for 3 h and analyzed by flow cytometry. RPE cells cultured without POS were used as controls (shown in red). e Transepithelial electrical resistance (TER) measurements of untreated and LbL-RPE cells cultured for 3 to 30 days were used to characterize epithelial barrier function (mean ± SEM; n = 6; p > 0.05)

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