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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Improving cell survival and engraftment in vivo via layer-by-layer nanocoating of hESC-derived RPE cells

Fig. 5

Biostability of the LbL coating and functional assessment of RPE and LbL-RPE cells from 2 to 5 weeks after transplantation. a Images of LbL-RPE cells after transplantation in RCS rats from 2 to 5 weeks, showing gelatin/alginate/gelatin-FITC-encapsulated RPE cells could effectively engraft around the grafted area. RPE cells received 3 layers of LbL coating; the last layer of gelatin was conjugated with FITC. Scale bar 20 μm. b The number of gelatin/alginate/gelatin-FITC-encapsulated RPE cells around the transplanted area at 2, 3, and 5 weeks after transplantation (bars: mean ± SEM; n = 3; **p < 0.01). c Immunofluorescence staining observed by confocal microscopy showed colocalization of human mitochondria and Hoechst in gelatin/alginate/gelatin-FITC-encapsulated RPE cells at 5 weeks after transplantation. Scale bar 20 μm. d Representative average scotopic ERG traces obtained from RCS rats at 2 and 5 weeks after transplantation (n = 8 rats per condition). e The average B wave amplitude is well maintained in animals with LbL-RPE cell grafts compared to grafts containing untreated RPE cells and suggests some maintenance of visual function as a result (mean ± SEM.; **p < 0.01)

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