Fig. 6

Survival assessment of RPE and LbL-RPE cells in vivo. a–d Immunofluorescence staining observed by confocal microscopy. Transplanted cells (pre-labeled with Dil (red)) expressed human mitochondria (green) or RPE65 (green) markers. Transplanted LbL-RPE cells remained at the injection site at 5 and 21 weeks after surgery (arrowhead). Only a limited number of untreated RPE cells remained in graft area at 5 and 21 weeks after surgery (arrowhead). Arrows indicated viable transplanted RPE and LbL-RPE cells which were human mitochondria or RPE65, Hoechst and Dil positive. Scale bar 20 μm. e, f Quantitation showing the number of cells labeled with the human mitochondrial or RPE65 antibody at 5 and 21 weeks after RPE and LbL-RPE cell transplants (mean ± SEM; ***p < 0.001; **p < 0.01; n = 8)