Fig. 2

Intra-donor mean (+ SD) cultivation times of mixed (unsegregated) cell cultures. a Mean cultivation times during cell expansion from culture initiation until passage 6 shown by passage (left panel) and in total (right panel). Black dotted lines indicate passage means. b Mean duration of subcultivation (from 4 to 16 × T175 culture flasks) before isolation of the ABCB5⁺ cells from fresh (left panel) and from previously cryopreserved/thawed cultures (right panel) for up to four isolation cycles. Fresh cultures: isolation cycles 1–4 correspond to passages 7–10; thawed cultures: isolations cycles 1–3 correspond to passages 9–11. Data represent all expansions and isolation cycles performed between January 2018 and September 2019; therefore, for donors 1 and 2, only thawed cultures are shown (because expansion and isolation from fresh cultures had been performed before January 2018). Red dashed lines indicate the upper time limit; if a culture had not reached target confluency within this prespecified period, it would have been discarded