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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Process data of allogeneic ex vivo-expanded ABCB5+ mesenchymal stromal cells for human use: off-the-shelf GMP-manufactured donor-independent ATMP

Fig. 4

Lab-assistant-specific performance of ABCB5+ cell isolation. Performance is expressed as the mean percentage (± SD) from the mean live cell count of all ABCB5+ cell isolates that were afterwards pooled to the same drug substance batch. Cell counting was performed strictly independently from the lab assistant by the quality control department using a standardized automated method (flow cytometry). Included in this analysis are all lab assistants who had carried out at least 10 isolations (n = 721 isolations in total), with the less experienced lab assistants (10–19 isolations) colored in light blue and the more experienced (≥ 20 isolations) in median blue. *p < 0.05 (one-way ANOVA with Bonferroni’s post hoc test) vs. the most experienced assistant (G, dark blue). Note that the letters A–N identifying the lab assistants are arbitrarily assigned based on the order of data arrangement and do not match with those used in Fig. 6

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