Fig. 6

Downregulation of SNHG5 inhibits TGF-β1-mediated HESC fibrosis and the Wnt/β-catenin signalling pathway. a Relative expression of SNHG5 mRNA after si-SNHG5 transfection into primary HESCs. Measurement data are expressed as the means ± SEM, n = 3; *P < 0.05, **P < 0.01, one-way ANOVA. b Relative FOXF2 mRNA expression after si-SNHG5 and sh-SNHG5 transfection into primary HESCs. Measurement data are expressed as the means ± SEM, n = 3; *P < 0.05, **P < 0.01, ***P < 0.001, one-way ANOVA. c Relative SNHG5 mRNA expression after si-FOXF2 transfection into primary HESCs. Measurement data are expressed as the means ± SEM, n = 3; ***P < 0.001, one-way ANOVA. d Relative expression of mRNAs in primary HESCs transfected with si-SNHG5 before and after TGF-β1 treatment. The measurement data are expressed as the means ± SEM, n = 3; *P < 0.05, **P < 0.01, ***P < 0.001, one-way ANOVA. e Protein expression in primary HESCs transfected with si-SNHG5 before and after TGF-β1 treatment. f IF showing the protein expression levels of FOXF2 and β-catenin in primary HESCs transfected with si-SNHG5 before and after TGF-β1 treatment. g Flow cytometry analysis showing changes in the cell cycle of primary HESCs transfected with si-SNHG5 before and after TGF-β1 treatment