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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Bone marrow mesenchymal stem cell-derived exosomes promote plasminogen activator inhibitor 1 expression in vascular cells in the local microenvironment during rabbit osteonecrosis of the femoral head

Fig. 3

Characterization of BMMSCs and significantly upregulated PAI-1 affected by GC treatment in vitro. a Representative BMMSCs exhibited a typical spindle-shaped morphology with or without GC-treatment. Magnification: ×200. b Flow cytometry analysis of the BMMSC cell surface markers (positive for CD90 and CD44 and negative for CD34 and CD45). c Representative alkaline phosphatase staining (top) and oil red O staining (bottom) of BMMSCs from the control and GC-treated groups. Magnification: ×200. d Expression of PAI-1 in BMMSCs was significantly increased following treatment with GC, lipopolysaccharide, and a combination of the two, as shown by qRT-PCR. e Expression of PAI-1 in BMMSCs was significantly increased following treatment with any concentration of GC, as shown by qRT-PCR and western blotting. Asterisks (*) indicate significant differences (p < 0.05 by ANOVA). The results are presented as the means ± s.e.m

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