Fig. 6

Exosomal microRNAs from BMMSCs regulated PAI-1 expression in vascular cells. a Venn diagram illustrating the overlap of DEGs by miRNA sequencing. b Volcano plot of global miRNA changes with specks denoting genes with significant fold changes (green specks for downregulated miRNAs and red specks for upregulated miRNAs). c A significant increase of miR-451-5p expression and a significant reduction of miR-133b-3p expression in GB-Exo groups were detected by qRT-PCR. d Predicted secondary structure of the miR-133b-3p precursor. The whole sequence is the precursor of miRNAs, and the red prominent part is the mature body sequence. e The binding sites for miR-133b-3p within the PAI-1 gene sequence (SERPINE1). f qRT-PCR results demonstrated that the expression levels of PAI-1 were reduced by treatment with miR-133b-3p mimics and increased by treatment with an miR-133b-3p inhibitor in VECs, which was also verified by western blotting (g). Asterisks (*) indicate significant differences (p < 0.05) and **p < 0.01. The results are presented as the means ± s.e.m