Fig. 3
From: Skeletal muscle healing by M1-like macrophages produced by transient expression of exogenous GM-CSF
uP-mGM transfection promotes decreased fibrosis and alters the fibrosis-related gene expression profile. a Micrographs of TA skeletal muscle tissue stained with Picrosirius Red and histomorphometry carried out using 20 images within the injured area per slide of each animal after staining. Data are presented in % (mean ± SD) of each parameter/total analyzed area. Bar = 50 μm. Two-way ANOVA and Tukey’s post hoc test. *p < 0.5, ***p < 0.001. b Expression of TGF-β1 and TGF-β3 was assessed by IHC, and histomorphometry was carried out using 20 images per slide of each animal after staining. Bar = 50 μm. Two-way ANOVA and Tukey’s post hoc test. **p < 0.1, ***p < 0.001. c Fibrogenic gene expression analyzed by RT-qPCR. Two-way ANOVA and Tukey’s post hoc test. “a” p < 0.05 LM vs LM+GM; “b” p < 0.05 LM vs GM; “c” p < 0.05 LM+GM vs GM