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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Skeletal muscle healing by M1-like macrophages produced by transient expression of exogenous GM-CSF

Fig. 4

uP-mGM transfection promotes increased GM-CSF receptor expression and vessel density. a Micrographs of TA skeletal muscle tissue stained with an α-smooth muscle actin (α-SMA) antibody. Histomorphometry was carried out using 20 frames within the injured area per slide of each animal after staining. Bar = 20 μm. Two-way ANOVA and Tukey’s post hoc test. ***p < 0.001. Arrow heads indicate large vessels stained with α-SMA. b Micrographs of the same sample stained with Isolectin IB4 Biotin. Histomorphometry was carried out using 20 frames within the injured area per slide of each animal after staining. Bar = 20 μm. Two-way ANOVA and Tukey’s post hoc test. *p < 0.5, ***p < 0.001. Arrow heads indicate capillaries. c Angiogenic gene expression analyzed by RT-qPCR. Two-way ANOVA and Tukey’s post hoc test. “a” p < 0.05 LM vs LM+GM; “b” p < 0.05 LM vs GM; “c” p < 0.05 LM+GM vs GM

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