Fig. 5

uP-mGM transfection leads to increased numbers of the M2 macrophage subtype. a Flow cytometry analysis of the macrophage subpopulations. Gating strategy used to identify M0 (CD45⁺ F4/80⁺ CD206⁻ MHCII⁻), M1 (CD45⁺ F4/80⁺ CD206⁻ MHCII⁺), M2 (CD45⁺ F4/80⁺ CD206⁺ MHCII⁻), and M1/M2 (CD45⁺ F4/80⁺ CD206⁺MHCII⁺) cells. b Macrophage subpopulations in the LM, GM, and LM+GM groups over 15 days. The normal group represents mice that did not undergo injury and treatment. c Variation in the numbers of macrophages per subpopulation over time. Time zero is represented by macrophages from the normal group, as mentioned above (b). Two-way ANOVA and Tukey’s post hoc test. “a” p < 0.05 LM vs LM+GM; “b” p < 0.05 LM vs GM; “c” p < 0.05 LM+GM vs GM