Fig. 5
hPMSCs upregulate the expression of Nrf2 of senescent CD4+ T cells. 4 × 106 naive CD4+ T cells (CD4CD45RA cells) were isolated using immunomagnetic beads and pretreatment for 1 h with Akt pathway inhibitor LY294002 (30 μM). Then, hPMSCs were added at a 1:10 ratio to CD4+ T cells and co-cultured for 72 h in the presence or absence of anti-CD3/CD28 Dynabeads and IL-2 as a mitogenic stimulus. a The scheme showing the experimental design. b Protein levels of Nrf2 and its downstream target genes NQO1, HO-1, CAT, and GCLC. c The level of total Nrf2. d The ratio of nuclear/cytoplasm Nrf2. e The mRNA expressions of Nrf2 in CD4+ T cells. f–i The protein level of Nrf2 downstream target genes GCLC, HO-1, CAT, and NQO1 in CD4+ T cells. j–m The mRNA expressions of GCLC, HO-1, CAT, and NQO1 in CD4+ T cells. Data represent the mean scores ± SEM of at least three independent experiments. *p < 0.05, **p < 0.01