Fig. 1

Differentiation of three human iPSC lines toward HEPs using the GSK3β inhibitor CHIR99021. a Schematic diagram of the differentiation protocol. The medium was changed daily during differentiation. b Representative images of differentiated MUSIi011-A cells from day 0 to 5 (scale bar = 200 μm). c Representative flow cytometric analysis shows the expression of HEP markers at day 3 and day 5 of differentiation. The KDR⁺CD235a⁻ cells were gated for analysis of HEP markers (KDR⁺CD34⁺CD31⁺). d Percentage of ME cells (KDR⁺CD235a⁻CD34⁻) and HEPs (KDR⁺CD34⁺CD31⁺) on day 3 and day 5. Data were obtained from at least three independent experiments (N ≥ 3). Error bars represent mean ± SEM. e Quantitative PCR analysis of pluripotent (NANOG, OCT4), mesendodermal (Brachyury), and mesodermal (KDR) genes at day 0, day 3, and day 5 of differentiation. Data were obtained from three independent experiments (N = 3). Error bars represent mean ± SEM