Fig. 3

HSPC differentiation of the day 5 HEPs derived from the MUSIi011-A cells. a Schematic diagram of HSPC differentiation from iPSCs. b Representative images of differentiated cells on day 5 to day 12 (scale bar = 200 μm). c Representative flow cytometric results of HSPC markers, CD34, and CD43, at day 8 and day 12 of differentiation. d Percentages of subpopulations, CD34⁺CD43⁻, CD34⁺CD43⁺, and CD34⁻CD43⁺ cells at day 8 and day 12 of differentiation. Data are presented as mean ± SEM from five independent experiments. e Representative images of hematopoietic colonies from CFU assay. Day 8 floating cells were seeded in MethoCult™ medium for 2 weeks (scale bar = 200 μm)