Fig. 4
From: Activation of mesenchymal stem cells promotes new bone formation within dentigerous cyst
Bm-DCSCs and Am-DCSCs exhibited a multi-differentiation potential. a Oil Red O staining. b Alcian blue staining. c Alkaline phosphatase staining (ALP) and Alizarin Red staining (ARS) at different osteogenic induction time. Relative activity of ALP and ARS were detected at the same time. ****p < 0.0001. d Real-time polymerase chain reaction (RT-PCR) for messenger RNA (mRNA) expression levels of runt-related transcription factor 2 (Runx2) and osteocalcin (OCN). ****p < 0.0001. e Immunofluorescence staining for OCN and RUNX2 on Bm-DCSCs and Bm-DCSCs after 7 days’ osteogenic differentiation. Scale bars: 20 μm. The undifferentiated cells were shown at the lower left corner in each image with a magnification of × 1000