Fig. 3

MCPs can promote the neuronal differentiation of C17.2-NSCs under pathological conditions. a C17.2-NSCs were treated with glutamic acid and then perfused of different periods (0 days, 1 day, 3 days, 5 days, 7 days, 14 days). Western blots for TUJ1, GFAP, and CNP from indicated group. b Data were presented as the relative density of TUJ1, GFAP, and CNP compared with that of β-actin. c C17.2-NSCs were treated with different concentration of MCP (2.0, 5.0, 10.0 μg/ml) after glutamic acid stimulation reperfusion of 3 days (Glu/3d+MCP group). Western blots for TUJ1, GFAP, and CNP from indicated group. d Data were presented as the relative density of TUJ1, GFAP, and CNP compared with that of β-actin. e Immunofluorescent staining of TUJ1 (red), GFAP (red), and CNP (green) in the indicated group. DAPI was used to stain the cell nucleus (blue). Scale bar, 100 μm. Data were given as mean ± SD according to ANOVA with n = 3 independent experiments in triplicate per group. ^aP < 0.05 vs. control, ^bP < 0.05 vs. Glu/3d group