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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Momordica charantia polysaccharides modulate the differentiation of neural stem cells via SIRT1/Β-catenin axis in cerebral ischemia/reperfusion

Fig. 4

Activation of SIRT1 inducing by MCPs is responsible for the neuronal differentiation of C17.2-NSCs. a Western immunoblots of SIRT1 from indicated group. b Data were presented as the relative density of SIRT1 compared with that of β-actin from indicated group. c Activity of SIRT1 in C17.2-NSC differentiation by SIRT1 activity assay. d Western blots for TUJ1, GFAP, and CNP from indicated group. e Data were presented as the relative density of TUJ1, GFAP, and CNP compared with that of β-actin. f Immunofluorescence staining of TUJ1 (green) and DAPI (blue) in C17.2-NSCs from indicated group. Scale bar, 100 μm. g Quantitative analysis showing the percentage of TUJ1+ cells in indicated group. Data were given as mean ± SD according to ANOVA with n = 3 independent experiments in triplicate per group. aP < 0.05 vs. Con, bP < 0.05 vs. MCP group

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Stem Cell Research & Therapy

ISSN: 1757-6512