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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Momordica charantia polysaccharides modulate the differentiation of neural stem cells via SIRT1/Β-catenin axis in cerebral ischemia/reperfusion

Fig. 5

MCP-induced deacetylations via SIRT1 promote nuclear accumulation of β-catenin in C17.2-NSCs. a Western immunoblots of Ac-β-catenin after glutamic acid stimulation reperfusion of indicated time point. b Quantitative analysis of the expression of Ac-β-catenin compared with that of β-actin from indicated group. c Fractionated extracts analysis. Western immunoblots of β-catenin in nuclear and cytoplasmic from indicated group. d, e Statistical analysis for β-catenin nuclear accumulation. β-actin or LaminB1 was used for signal normalization. f, h Western immunoblots of the nuclear accumulation of β-catenin and Ac-β-catenin (IP, acety Lys; IB, β-catenin) from indicated group in glutamate-treated C17.2-NSCs. g Quantitative analysis of the nuclear accumulation of β-catenin and Ac-β-catenin treatment with RSV or NA (f). i Quantitative analysis of the nuclear accumulation of β-catenin and Ac-β-catenin treatment with MCPs or SIRT1-siRNA (h). Data were given as mean ± SD according to ANOVA with n = 3 independent experiments in triplicate per group. aP < 0.05 vs. Con, bP < 0.05 vs. RSV25μM or MCP group

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