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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Enhanced differentiation of human pluripotent stem cells into pancreatic endocrine cells in 3D culture by inhibition of focal adhesion kinase

Fig. 2

Comparison of pancreatic endocrine cells derived from 2D and 3D cultures. a qRT-PCR analysis of pancreas-specific transcriptional factors (PDX1, NKX6.1, and NGN3), endocrine cell-specific transcription factors (PAX6 and ISL1), and pancreatic hormones (INS, GCG, and SST) in EPs-2D, EPs-3D, PECs-2D, and PECs-3D. b Immunostaining of insulin (INS), glucagon (GCG), and somatostatin (SST) in PECs induced under 2D or 3D culture conditions and fetal or adult islets. c Flow cytometry analysis of INS in PECs-2D or PECs-3D. Data represent mean ± SD (n = 3), *p < 0.05, **p < 0.01, ***p < 0.001

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Stem Cell Research & Therapy

ISSN: 1757-6512