Fig. 5

SMF activates RUNX2-mediated signaling in BMSCs in osteogenesis differentiation medium. a Heatmap of mRNA expression of osteogenic genes, as detected by deep sequencing, in BMSCs cultured in osteogenesis differentiation medium and stimulated with 0.6 T SMF for 48 h (fold change > 2, false discovery rate < 0.20). b Western blot analysis of RUNX2 and ALP in SMF-exposed BMSCs cultured in osteogenesis differentiation medium. c GO pathway enrichment analyses of significantly upregulated genes in SMF-exposed BMSCs grown in osteogenesis differentiation medium. Top 20 enriched pathways are shown.*P<0.05. d GSEA indicates that osteogenesis-positively correlated genes are significantly enriched in the SMF-exposed BMSCs compared with those in control cells