Fig. 1
Processing of adipose tissue. Lipoaspirates are collected using draining agents and undergo mechanical and/or enzymatical procedure to separate the stromal vascular fraction (SVF). ADSCs are then isolated mostly by seeding SVF in culture and expanded before releasing to the point of care. Cryopreservation ensures storage in liquid nitrogen before using in clinical issues. This manual flow chart has been improved by using all-in-one single-use kits and commercialized medical devises in completely closed or semi-closed systems to meet the good manufacturing practices (GMP) requirements for therapeutic use of stem cells and derivatives