Table 2 Primary and secondary antibodies list
Antibodies | Dilution | Company | |
|---|---|---|---|
Primary antibody | Rabbit beta-tubulin antibody, polyclonal | 1:10,000 | Abcam |
Primary antibody | Rabbit AKT antibody, polyclonal | 1:1000 | Cell signaling |
Primary antibody | Rabbit phospho-AKT (Ser473) antibody, polyclonal | 1:1000 | Cell signaling |
Primary antibody | Rabbit anti-FoxO1 antibody, monoclonal | 1:1000 | Abcam |
Primary antibody | Rabbit EGF antibody, polyclonal | 1:500 | Bioss |
Primary antibody | Rabbit anti-FOXA2 antibody, monoclonal | 1:1000 | Abcam |
Primary antibody | Rabbit anti PDX-1 antibody, polyclonal | 1:500 | Merck |
Secondary antibody | Polyclonal Goat Anti-Rabbit Immunoglobulins/HRP | 1:3000 | Dako |