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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Human adipose-derived stem cells enriched with VEGF-modified mRNA promote angiogenesis and long-term graft survival in a fat graft transplantation model

Fig. 1

Efficacy and kinetics of modRNA transfection in hADSCs. ac Transfection efficiency and the expression kinetics of modGFP in hADSCs. a Representative images depicting GFP signal in hADSCs at 4, 8, 16, 24, and 48 h post-transfection. b Flow cytometry analysis of transfection efficiency at 4, 8, 16, 24, and 48 h post-transfection. c Flow cytometry analysis of mean fluorescence signal intensity at 4, 8, 16, 24, and 48 h post-transfection. df Expression levels of d VEGF mRNA and ef VEGF protein at 24 h post-transfection. gh Kinetics of g newly produced and h cumulative VEGF protein concentrations periodically monitored for several days following transfection of modVEGF in hADSCs. Scale bar = 100 μm. Error bars showed means ± SD. (n = 3; *p† < 0.05, **p† < 0.01, ***p† < 0.001, ****p† < 0.0001)

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