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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Human adipose-derived stem cells enriched with VEGF-modified mRNA promote angiogenesis and long-term graft survival in a fat graft transplantation model

Fig. 7

modVEGF-engineered hADSCs reduce levels of fibrosis, apoptosis, and necrosis in fat grafts. Fat grafts were harvested at 90 days after grafting for detailed molecular assessment. a Representative Masson’s trichrome staining of fat grafts. b Quantification of collagen (% area). Fibrotic areas are highlighted in blue with regions of extensive collagen deposition. c Representative immunostainings of α-SMA and CD31 shows mature blood vessels. d Analysis of α-SMA+ and CD31 adipocytes (%). e Analysis of α-SMA+ and CD31+ vessels (%). Note: the hADSCmodVEGF group presented the least fibrotic tissue and gave rise to the most SMA+ capillaries, an indication of mature blood vessel formation. f Visualization of TUNEL+ cells indicates apoptotic activity. g Quantification of apoptotic cells. h Representative photomicrographs of TNF-α+ stainings. Black arrowheads practically indicate necrotic cells. i Analysis of TNF-α+ area (%). Scale bars = 20 μm. Error bars showed means ± SD. (*p† < 0.05, **p† < 0.01, ***p† < 0.001)

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