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Fig. 8 | Stem Cell Research & Therapy

Fig. 8

From: Human adipose-derived stem cells enriched with VEGF-modified mRNA promote angiogenesis and long-term graft survival in a fat graft transplantation model

Fig. 8

modVEGF-engineered hADSCs retain higher implantation viability and differentiation capacity to support a long-term survival of fat grafts. hADSCs engineered with modLuc or modVEGF were labeled with CM-DiI before mixing with fat and undergoing transplantation. Fat grafts were harvested at 90 days after grafting for histological evaluation. a Representative images of CM-DiI-labeled hADSCs in fat grafts. b Ratio of CM-DiI-labeled hADSCs. c Representative immunostainings of CD31- and CM-DiI-labeled cells identify vascular endothelial cells differentiated from hADSCs. d Percentage of CD31+ and CM-DiI+ cells in total CM-DiI+ cells (%). e Visualization of perilipin+ and CM-DiI+ cells indicates adipocytes differentiation activity from hADSCs. f Percentage of perilipin+ and CM-DiI+ cells in total CM-DiI+ cells (%). Note: the hADSCmodVEGF group presented with more viable hADSCs in fat grafts and gave rise to more capillaries and differentiated adipocytes. Scale bar = 20 μm. Error bars showed means ± SD. (*p† < 0.05, **p† < 0.01)

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