Fig. 5

ABs promoted macrophage polarization towards the M2 phenotype. a The immunofluorescence images of time-dependent uptake of ABs by macrophages. Scale bar, 100 μm. b The immunofluorescence images of concentration-dependent uptake of ABs by macrophages. Scale bar, 100 μm. c The immunofluorescence images and quantification of the CD206 expression level of macrophages treated with different concentrations of ABs. Scale bar, 100 μm. d, e The detection of secretory factors IL-10 (d) and TGF-β (e) released by macrophages treated with different concentrations of ABs by ELISA. f The protein levels of ARG-1 and TGF-β of macrophages treated with different concentrations of ABs examined by western blots. g–i The gene expression level of Il-10 (g), Arg-1 (h), and Tgf-β (i) of macrophages treated with different concentrations of ABs examined by qPCR. n = 3 per group. Data shown as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001; NS, not significant