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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Definitive hematopoietic stem/progenitor cells from human embryonic stem cells through serum/feeder-free organoid-induced differentiation

Fig. 1

Two-step serum/feeder-free hematopoietic differentiation from human embryonic stem cells (ESCs) displayed high colony-forming unit (CFU) potential and generated a significant portion of hematopoietic stem cell (HSC)-like cells. 7-day embryoid body (EB) maturation followed by 8-day organoid differentiation provided a the lowest percentage of primitive (CD34− CD235a+) protocol and b the highest percentage of definitive (CD34+ CD235a−) progenitors. c Matrigel or gelatin coating did not result in a statistically significant difference in hematopoietic cell emergence. d CFU potential, e HSC-like, and f hemogenic endothelium cell (HE) fraction in different experimental groups (n = 3, *p < 0.05, **p < 0.01)

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