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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Definitive hematopoietic stem/progenitor cells from human embryonic stem cells through serum/feeder-free organoid-induced differentiation

Fig. 4

CD34+ CD43+ cells derived from human ESCs generate T-lymphocytes with a broad T cell receptor (TCR) repertoire. a Representative flow cytometry analysis of CD5 and CD7 expression in ESC-derived CD34+ CD43+ cells after 22 days of T cell differentiation on OP9/DLL1 co-culture. b Representative flow cytometry analysis of CD4, CD8, and CD3 expression in ESC-derived CD34+ CD43+ cells at 22 days of T cell differentiation on OP9/DLL1 co-culture. c The frequency of CD3+ cells in CD4+CD8+ double-positive T cells on day 22 and day 27. Values represent mean ± SD (n = 3). d TCR-Vβ deep sequencing showed the diversity of rearranged TCR genes in ESC-derived CD4+ CD8+ CD3+ T cells at day 27 of differentiation

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Stem Cell Research & Therapy

ISSN: 1757-6512