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Table 1 Hematopoietic stem/progenitor cell (HSPC) differentiation media composition

From: Definitive hematopoietic stem/progenitor cells from human embryonic stem cells through serum/feeder-free organoid-induced differentiation

Embryoid body (EB) serum-free media [18] Organoid differentiation [12]
IMDM (Sigma-Aldrich) 243 ml IMDM 390 ml
Ham’s F12 (Sigma-Aldrich) 243 ml Knockout serum replacement (Thermo Fisher) 100 ml
BSA (Roche) 2.5 g ITS (100×) 5 ml
Insulin-Transferrin-Selenium (ITS, 100×, Sigma-Aldrich) 5 ml Ascorbic acid 25 mg
Lipid mixture (100×, Thermo Fisher, Waltham, MA) 5 ml L-glutamine (100×) 5 ml
Ascorbic acid (Sigma-Aldrich) 25 mg 1-thioglycerol 19 μl
L-glutamine (100×, Thermo Fisher) 5 ml  
1-thioglycerol (Sigma-Aldrich) 19 μl