Embryoid body (EB) serum-free media [18] | Organoid differentiation [12] | ||
---|---|---|---|
IMDM (Sigma-Aldrich) | 243 ml | IMDM | 390 ml |
Ham’s F12 (Sigma-Aldrich) | 243 ml | Knockout serum replacement (Thermo Fisher) | 100 ml |
BSA (Roche) | 2.5 g | ITS (100×) | 5 ml |
Insulin-Transferrin-Selenium (ITS, 100×, Sigma-Aldrich) | 5 ml | Ascorbic acid | 25 mg |
Lipid mixture (100×, Thermo Fisher, Waltham, MA) | 5 ml | L-glutamine (100×) | 5 ml |
Ascorbic acid (Sigma-Aldrich) | 25 mg | 1-thioglycerol | 19 μl |
L-glutamine (100×, Thermo Fisher) | 5 ml | ||
1-thioglycerol (Sigma-Aldrich) | 19 μl |