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Table 1 Hematopoietic stem/progenitor cell (HSPC) differentiation media composition

From: Definitive hematopoietic stem/progenitor cells from human embryonic stem cells through serum/feeder-free organoid-induced differentiation

Embryoid body (EB) serum-free media [18]

Organoid differentiation [12]

IMDM (Sigma-Aldrich)

243 ml

IMDM

390 ml

Ham’s F12 (Sigma-Aldrich)

243 ml

Knockout serum replacement (Thermo Fisher)

100 ml

BSA (Roche)

2.5 g

ITS (100×)

5 ml

Insulin-Transferrin-Selenium (ITS, 100×, Sigma-Aldrich)

5 ml

Ascorbic acid

25 mg

Lipid mixture (100×, Thermo Fisher, Waltham, MA)

5 ml

L-glutamine (100×)

5 ml

Ascorbic acid (Sigma-Aldrich)

25 mg

1-thioglycerol

19 μl

L-glutamine (100×, Thermo Fisher)

5 ml

 

1-thioglycerol (Sigma-Aldrich)

19 μl