Skip to main content
Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Circ6401, a novel circular RNA, is implicated in repair of the damaged endometrium by Wharton’s jelly-derived mesenchymal stem cells through regulation of the miR-29b-1-5p/RAP1B axis

Fig. 3

circ6401 enhances the endometrium repair function of WJ-MSCs. a Structure of the pLC5-ciR vector which contained a front and back circular frame. b GFP expression and infection efficiency were determined under fluorescence microscopy to select the optimal MOI for maximal transgene expression. MOI of 50 was finally used. Scale bar, 50 μm. c Efficiency of infected circ6401-overexpressing WJ-MSCs detected by qRT-PCR. **P < 0.01. d The cell morphology of ESCs in the four groups. Scale bar, 500 μm. e Flow cytometry analysis was used to analyze ESC apoptosis after cocultured with lenti-circ6401+ WJ-MSCs or lenti-NC WJ-MSCs. f Quantitative analysis of flow cytometry analysis. Data in panel are shown as means ± standard deviation (n = 3). **P < 0.01. g VEGFA, VEGFR1, VEGFR2, and RAP1B protein expression in lenti-circ6401+ WJ-MSCs, lenti-circ6401− WJ-MSCs by western blot assay. h Quantitative analysis of western blot assay. Data in panel are shown as means ± standard deviation (n = 3). **P < 0.01

Back to article page