Fig. 3
Epithelial integrity and metabolic activity of FDLE cells were not affected by MSC-CM. FDLE cells were subjected to MSC-CM or control medium for 24 h. Data are displayed as mean + SEM or box and whiskers with the 10–90 percentiles, mean (+) and median (horizontal line). Statistical differences between the two groups were analyzed with a t test. a Epithelial integrity was determined with FITC-dextran assays. MSC-CM had no effect on the permeability of FDLE monolayers (n = 8, 2 IE). b Rte was not altered by MSC-CM, determined in Ussing chambers (n = 28–31, 2 IE). c No altered metabolic activity was observed in MSC-CM-treated FDLE cells by MTT assays (n = 6, 2 IE). □ control, ■MSC-CM