Fig. 4
Growth factors were not responsible for the stimulating effect of MSC-CM on Na+ transport. FDLE cells were subjected to MSC-CM or control medium for 24 h with or without the respective growth receptor inhibitor. Data are displayed as boxes and whiskers with the 10–90 percentiles, mean (+), and median (horizontal line). Statistical differences among groups were analyzed with an ANOVA and Tukey’s post hoc test. a Inhibition of VEGF-R with Axitinib (n = 19–23, 2 IE; ***p < 0.001), b BMP-R with K02288 (n = 22–24, 2 IE; ***p < 0.001), and c PDGF-R with AG-1296 (n = 18–22, 2 IE; ***p < 0.001) did not affect Na+ transport in MSC-CM-treated and control cells. d The EGF-R inhibitor AG-1478 (n = 19–24, 2 IE; ***p < 0.001), e the TGF-β-R inhibitor SB431542 (n = 21–24, 2 IE; *p < 0.05; ***p < 0.001), f the FGF-R inhibitor FIIN-2 (n = 16–24, 2 IE; *p < 0.05; **p < 0.01; ***p < 0.001), and g the HGF-R (c-met) inhibitor PHA665752 (n = 64–67, 6 IE; **p < 0.01; ***p < 0.001) reduced ∆Iamil in control and MSC-CM-treated cells. Inhibition did not prevent the stimulating effect of MSC-CM. □ control, ■MSC-CM