Fig. 5

Inhibition of kinase signaling decreased MSC-CM-mediated Na⁺ channel activity. FDLE cells were subjected to MSC-CM or control medium for 24 h with or without the respective kinase inhibitor. Data are displayed as boxes and whiskers with the 10–90 percentiles, mean (+) and median (horizontal line). Statistical differences among groups were analyzed with an ANOVA and Tukey’s post hoc test. a ∆I_amil was significantly reduced in both, MSC-CM-treated and control FDLE cells, when Akti 1/2 was present in the culture medium. MSC-CM was not able to increase ∆I_amil anymore (n = 17–19, 2 IE; ***p < 0.001). b Akti 1/2 did not affect R_te of FDLE cells. c ∆V_amil of both control and MSC-CM-treated cells was decreased by Akti 1/2. AKT inhibition further prevented the increase of ∆V_amil induced by MSC-CM (***p < 0.001). d LY294002-mediated inhibition of PI3-K did not affect ∆I_amil in MSC-CM-treated FDLE cells (n = 42–50, 8 IE; ***p < 0.001). e LY294002 significantly decreased R_te of FDLE cells cultured in MSC-CM (**p < 0.01). f ∆V_amil of MSC-CM-treated cells was decreased by LY294002, which further prevented the MSC-CM-induced increase of ∆V_amil (***p < 0.001). □ control, ■ MSC-CM